Mogroside V Determination by HPLC with Charged Aerosol and UV Detections

Extracts of luo han kuo fruit used as sweeteners were acknowledged as Generally Recognized as Safe (GRAS) based on a GRAS submission to the U.S. FDA in January of 2010.2 Typical reversed-phase high-performance liquid chromatography (HPLC) methods to determine these glycosides are challenging due to the lack of a strong, specific chromophore in the compound.

In this application note, mogroside V is determined in a luo han kuo beverage by both charged aerosol and UV detections. This triterpene glycoside is separated on the Thermo Scientific Acclaim Trinity P1 column using 81/19 acetonitrile/ ammonium formate buffer at pH = 3.0. The developed method uses hydrophilic interaction liquid chromatography (HILIC) conditions suitable for the trimode column, allowing separation of multiple terpene glycosides. The same method has also been used to separate steviol glycosides.