Distinguishing Oxidative Impurities From Ionizable Lipids Used in LNP Formulations

Lipid nanoparticles (LNPs), comprised of ionizable lipids, are used to deliver oligonucleotides to work as therapeutics or to stimulate the immune system, as in the initial mRNA-based COVID vaccines. To ensure product quality, detailed and sensitive characterization of the ionizable lipid and its related impurities is necessary. However, obtaining the level of detail needed is challenging with current liquid chromatography-mass spectrometry (LC-MS)-based methodologies.

Download this app note to learn how electron activated dissociation can provide:

• Detection and identification of lipid impurities at levels below 0.01% relative abundance of the main peak
• Information-rich MS/MS spectra for definitive and distinct structural elucidation
• Improved risk assessment of raw material and formulated LNPs