净化的合成Oligodeoxynucleotides通过捕捉聚合

最近,大量的纯合成oligodeoxynucleotides (ODNs)需要临床前研究,病人使用,和临床试验。这些ODNs合成在一个自动化的合成器。通常,ODNs产生杂质的合成包括故障序列,这是很难去除。原因是他们有相同的属性完整ODNs。目前,ODNs净化技术可以去除这些杂质,如反相高效液相色谱法(RP HPLC)、阴离子交换高效液相色谱法,聚丙烯酰胺凝胶电泳(页面)。然而,所有这些方法都是不方便或昂贵的扩大。要解决这些问题,两个non-chromatographic发达ODNs净化的聚合方法。在第一种方法,在自动合成,全身ODNs标记与甲基丙烯酰胺组通过一个可分裂的序列不链接器而失败。长篇ODNs纳入一个聚合物通过共聚过程和失败序列和其它杂质被洗涤。长篇ODNs是通过从聚合物裂开。 In the second method, the failure sequences are capped by a methacrylated phosphoramidite followed by radical acrylamide polymerization and water extraction to retrieve the full-length ODNs. The purity of ODNs has been confirmed by RP HPLC. The identity of ODNs has been established by comparing with authentic ODNs and Matrix Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) analysis. These methods do not require any expensive equipment and materials. Therefore, they are useful for large-scale drug ODN purification.