描述的prehaustorial抗叶锈病(柄锈菌triticina f . sp. tritici)在单粒小麦(小麦属植物monococcum)的大规模分析cDNA结束(梅斯)

叶锈病造成柄锈菌triticina f . sp. tritici是最常见的小麦锈病,导致全球高收益的损失。小麦属植物monococcum登记入册是有价值的资源为提高六倍体小麦抗叶锈病。在广泛的筛选程序加入t . monococcum Pi272560已被确认显示prehaustorial抗叶锈病。第一个实验显示,有效防御反应与氧化酵素的活动增加和发病相关的基因。这个种族特异性(水平)prehaustorial阻力阻止感染前吸器母细胞的形成。因此我们的研究的目标是(我)来分析生物化学背景的显微镜和测量电阻的叶子和过氧化氢含量(2)来确定分子背景通过全基因组表达的研究使用的大规模分析cDNA结束(梅斯)。二甲酚橙试验显示大量的过氧化氢1.88µM g - 1在接种叶12级后48小时内接种(hai)加入耐药。为了分析基因的表达导致观察到的防御反应,梅斯的RNA样品被孤立在第一个24海从抗性和敏感t monococcum加入了。段的时间内从主海120950标签,分裂到8 - 16个海之间95147标签,90150 - 24之间的海标签可以注释Swissprot数据库。使用计数每百万确定标签(cpm) 8海423,523年分裂到8 - 16个海之间,海552 - 24之间的标签确定差异表达。 These tags have been blasted to the NCBI database and differentially expressed peroxidases (between 9-31), chitinases (2-13), kinases (32-80) and pathogenesis related genes (0-18) were determined. The results show that higher or exclusive expression of peroxidases, chitinases and pathogenesis related genes are involved in prehaustorial resistance. In addition seven genes related to (leaf rust) resistance genes were detected. Based on the analysis of 1136 tags differentially expressed comprising 4358 SNPs, 362 differentially expressed SNP containing genes were mapped in silico using the Genomezipper.