改进RNA-Seq分析的分子索引

在本应用说明中，bioscientic公司和细胞研究公司介绍了一种新的RNA-Seq高精度基因表达分析试剂盒。我们证明NEXTflex™qRNA-Seq™Kit可以直接替换到配对端文库制备中，而不影响传统的RNA-Seq分析方法。This new kit efficiently generates libraries equivalent to standard RNA-Seq libraries with sample barcodes, but with the added feature of molecular indexing.  The value of using the added molecular indexing feature depends upon experimental design, data objectives, and sequencing depth for a given library complexity.