幼儿肠道微生物群与健康状态变量之间的相关性

目的:本研究探讨了极低出生体重儿蹒跚学步期肠道微生物群与健康状况变量之间的相关性。方法:该研究获得了大学机构审查委员会的批准，并获得了母亲的知情同意。我们在新生儿重症监护室(NICU)住院的6周内随访了VLBW婴儿(<1500克)，并在18名学步儿童2-3岁时进行了家访和粪便收集。我们分析了幼儿2-3岁时收集的肠道微生物群与健康变量(血红蛋白、出生后第一年的患病频率和固体食物的引入)之间的关系。对这些儿童2-3岁(平均年龄=X个月)的18个粪便样本进行了测序。使用前将粪便拭子样本保存在-80℃。使用PowerSoil-htp 96 Well Soil DNA Isolation Kit (MoBio)提取微生物基因组DNA。DNA样本被转发到杰克·吉尔伯特博士的实验室(阿贡国家实验室和芝加哥大学)。用16SrRNA测序分析微生物含量。使用Illumina MiSeq平台对16S rRNA V4区(515F-806R)进行PCR并测序，每个样品产生约100,000个250 bp的成对末端reads。结果:该研究招募了18名VLBW婴儿，平均胎龄28.3周(范围25.3 - 32.4)，平均出生体重1115.06克(范围675-1485克)。 The gender distribution was equal. Length of NICU stay mean was 68.67 days, with a range of 22 to 123 days. At the toddler home visit, the OTU abundance of the x stool samples showed i a higher abundance of Firmicutes to Bacteroidetes . Hemoglobin level was inversely related to Actinobacteria abundance in toddlers (r=-.697, p=.001, R2=0.486). The remaining OTU abundances showed weak or non-significant correlations to Hgb. However, Firmicutes and Bacteroidetes showed a significant variability prediction. Hemoglobin level was related to Bacteroidetes abundance in toddlers (r=.479, p=.044, R2=0.229) and inversely related to Firmicutes abundance in toddlers (r=-.516, p=.028, R2=0.266).Frequency of illness during the first year of life, age and introduction of solid foods were related to Actinobacteria abundance. Actinobacteria phylum abundance was positively related to the frequency of fever in the first year of life,
(r =。609年,p = .007)。我们使用多元回归来评估出生后第一年的发烧频率、年龄和血红蛋白水平对放线菌丰度的独特作用。出生后第一年的发烧频率和血红蛋白水平与结果呈中度半偏相关:出生后第一年的发烧频率半偏相关为0.426，血红蛋白水平半偏相关为- 0.547。患儿年龄呈- 0.059的弱半偏相关。后来固体食物的引入与Tenericures (r=。804年,p =组织)。结论:人体肠道菌群中厚壁菌门、拟杆菌门和放线菌门是最丰富的门，健康成人中厚壁菌门和拟杆菌门占主导地位，母乳喂养的婴儿中放线菌门占主导地位。血红蛋白水平与肠道微生物群之间的相关性表明，某些种类的肠道细菌可以影响造血以及身体如何处理氧气。饮食也有可能在这种关系中发挥作用。肠道微生物群是生理和病理生理作用的重要调节因子。 Further research is necessary regarding children’s health and hemoglobin relationships with gut microbiome.