Re-Convolving the Compositional Landscape of Primary and Recurrent Glioblastoma Reveals Prognostic and Targetable Tissue States

Glioblastoma is an aggressive diffusely infiltrating neoplasm that spreads beyond surgical resection margins, where it intermingles with non-neoplastic brain cells. This complex microenvironment harbouring infiltrating glioma and non-neoplastic brain cells is the origin of tumour recurrence. Thus, understanding the cellular and molecular features of the glioma microenvironment is therapeutically and prognostically important.

Here, Dr. Al Dalahmah used single-nucleus RNA sequencing (snRNAseq) of primary and recurrent glioma to define three compositional ‘tissue-states’ rooted in cohabitation of cell-types and transcriptional states. All these states exhibited variable degrees of infiltration by glioma cells. Dr. Al Dalahmah's findings define a novel compositional approach to glioma-infiltrated tissue which allowed his team to discover prognostic and targetable features, paving the way to new mechanistic and therapeutic discoveries.

During this talk we cover:

• Cellular and molecular features of the glioma microenvironment
• Single-nucleus RNA sequencing (snRNAseq) of primary and recurrent glioma