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目前过敏原陷阱分析

食物过敏原的可靠分析的先决条件是确保遵守法律规定标签条例。这可以通过蛋白质或DNA进行检测。蛋白质检测的优点是直接检测过敏成分,目前可以使用免疫(ELISA /简单的快速测试)或质量spectrometry-based (MS)技术。DNA检测是间接的,但允许将食物过敏原的存在验证通过使用另一个标记。每种方法的优点和缺点在个案基础上,需要考虑。定性的简单快速测试非常适合食品行业,可以进行现场测试。ELISA定量,携带方便,灵敏度高。基于抗体的测试可能的加工食品有问题。女士展示了许多承诺,但是目前仍然耗时且复杂的执行。女士遇到类似的问题与加工食品和灵敏度矩阵和参数的依赖。 Thus, this technique is only occasionally used. PCR provides the highest specificity and, depending on the target sequence, a very good to good level of sensitivity. PCR is still affected by influence of processing and matrix related factors. All methods exhibit a relatively high level of measurement error due to natural variation and production-related changes in the molecules relevant in the process of detection. However, by means of laboratory-based analyses it is possible to calibrate for the allergen in question and thus be able to make reliable measurements using the methods which are already available.

满足作者
马丁•罗德
马丁•罗德
产品质量研究所
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Baidu